Nyob rau hauv ib txoj kev tshawb fawb tshaj tawm hauv Biocatalysis thiab Agricultural Biotechnology, cov kws tshawb fawb tau ua tiav siv cov txheej txheem kho cov noob qoob loo los tsim cov qos yaj ywm transgenic nrog txo qis ntawm α-solanine, steroidal glycoalkaloid uas tuaj yeem ua rau tib neeg thaum noj ntau heev. Qhov kev ua tiav no muaj peev xwm los hloov pauv kev cog qoob loo thiab txhim kho zaub mov kev nyab xeeb.
Qos yaj ywm, cov qoob loo tseem ceeb thoob ntiaj teb, ib txwm tsim ob qhov loj steroidal glycoalkaloids: α-solanine thiab α-chaconine. Thaum cov tshuaj no ua haujlwm raws li kev tiv thaiv ntuj tsim rau cov nroj tsuag, lawv tuaj yeem ua rau muaj kev phom sij rau kev noj qab haus huv thaum noj ntau dhau. Cov xwm txheej xws li raug lub teeb, kev puas tsuaj rau lub cev, thiab kev cia khoom tsis zoo tuaj yeem ua rau muaj kev nce ntxiv ntawm glycoalkaloids hauv cov qos yaj ywm, ua rau muaj kev phom sij rau kev noj qab haus huv ntxiv.
Txhawm rau hais txog qhov teeb meem no, cov kws tshawb fawb los ntawm Is Nrias teb tau ua haujlwm CRISPRi / dCas9-KRAB noob kho cov txheej txheem txhawm rau txhawm rau txhawm rau solanidine galactosyltransferase (sgt1) noob hauv cov qos yaj ywm. Los ntawm kev tsom mus rau cov noob tshwj xeeb no, cov kws tshawb fawb tuaj yeem txo qis qib ntawm α-solanine hauv cov qos yaj ywm transgenic, thaum tawm hauv cov qib α-chaconine tsis cuam tshuam. Lub hom phiaj no ua kom cov qos yaj ywm muaj kev nyab xeeb rau kev noj thaum khaws lawv cov txheej txheem tiv thaiv ntuj tiv thaiv kab tsuag thiab kab mob.
Qhov tseem ceeb, kev txheeb xyuas kev noj haus ntawm cov qos yaj ywm hloov kho cov noob no qhia tsis muaj kev hloov pauv tseem ceeb piv rau cov qos yaj ywm. Qhov no txhais tau hais tias cov qos yaj ywm transgenic tswj lawv cov khoom noj muaj txiaj ntsig thiab tag nrho cov khoom xyaw, ua rau lawv muaj txiaj ntsig thiab muaj kev nyab xeeb rau cov neeg siv khoom.
Qhov kev tshawb pom ntawm qhov kev tshawb fawb no muaj qhov cuam tshuam loj heev rau yav tom ntej ntawm kev cog qoob loo. Los ntawm kev ua tiav kev txo qis ntawm α-solanine los ntawm kev kho cov noob, cov kws tshawb fawb tau ua txoj hauv kev rau kev txhim kho cov qos yaj ywm ntau yam uas muaj kev nyab xeeb dua rau tib neeg noj. Qhov kev tawg no tsis yog tsuas yog hais txog qhov muaj feem cuam tshuam txog kev noj qab haus huv cuam tshuam nrog glycoalkaloids tab sis tseem qhib qhov muaj peev xwm tshiab rau kev txhim kho cov khoom noj khoom haus thiab tag nrho cov qos yaj ywm zoo.
Txoj kev tshawb nrhiav qhov kev tshawb pom twb tau ua rau muaj kev tshawb fawb ntxiv thiab kev loj hlob hauv thaj tsam ntawm cov noob qoob loo-edited qos yaj ywm. Cov kws tshawb fawb thiab cov kws paub txog kev ua liaj ua teb tam sim no ua haujlwm rau kev kho kom zoo thiab nthuav dav rau cov txiaj ntsig thawj zaug no, nrog rau lub hom phiaj ntawm kev tsim ntau yam ntawm cov noob qoob loo-edited qos yaj ywm ntau yam uas muab cov khoom zoo dua qub, suav nrog txo glycoalkaloid qib, txhim kho cov khoom noj khoom haus zoo, thiab muaj zog tiv thaiv kab tsuag. thiab kab mob.
Daim ntawv thov kev vam meej ntawm cov noob kho cov tswv yim los tsim cov qos yaj ywm transgenic nrog cov qib solanine tsawg sawv cev rau qhov tseem ceeb hauv cov qos yaj ywm. Qhov kev rhuav tshem no tsis yog tsuas yog hais txog qhov muaj feem cuam tshuam txog kev noj qab haus huv uas cuam tshuam nrog kev noj glycoalkaloid ntau dhau, tab sis tseem ua rau txoj hauv kev rau kev txhim kho cov qos yaj ywm muaj kev nyab xeeb thiab muaj txiaj ntsig zoo dua. Raws li kev tshawb fawb hauv cheeb tsam no txuas ntxiv mus, peb tuaj yeem tos ntsoov rau yav tom ntej uas cov qos yaj ywm kho tau ua lub luag haujlwm tseem ceeb hauv kev ua kom muaj kev ruaj ntseg zaub mov thiab txhawb kev noj qab haus huv.